1. Aminoacyl tRNA synthetases require one active site to link tRNA to amino acid, and another site with esterase activity to cleave off mis-attached amino acids.
  2. In pancreatic RNAse, two histidine residues act as a charge-relay pair to catalyse the acid-hydrolysis of the phosphate ester bonds of RNA. In carbonic anhydrase, three histidine residues bind a zinc ion, on which a hydroxide ion is bound.